Application
Confocal immunofluorescent analysis of GTF2H2C Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
GTF2H2C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A549,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the GTF2H2C antibody detected the GTF2H2C protein (arrow).| Product Name | GTF2H2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | General transcription factor IIH subunit 2-like protein, General transcription factor IIH polypeptide 2-like protein, GTF2H2C |
| Product description | Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II (By similarity).1) Schmutz, J., et al. Nature 431(7006):268-274(2004) |
| Immunogen | This GTF2H2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human GTF2H2C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2H2C |
|---|---|
| Alternative Names | General transcription factor IIH subunit 2-like protein General transcription factor IIH polypeptide 2-like protein GTF2H2C |
| Molecular Weight(MW) | 44 kDa |
Application
Confocal immunofluorescent analysis of GTF2H2C Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
GTF2H2C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A549,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the GTF2H2C antibody detected the GTF2H2C protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
