The product of this gene is a zinc finger protein with
nine Cis[2]-His[2] zinc finger domains. It functions as an RNA
polymerase III transcription factor to induce transcription of the
5S rRNA genes. The protein binds to a 50 bp internal promoter in
the 5S genes called the internal control region (ICR), and
nucleates formation of a stable preinitiation complex. This complex
recruits the TFIIIC and TFIIIB transcription factors and RNA
polymerase III to form the complete transcription complex. The
protein is thought to be translated using a non-AUG translation
initiation site in mammals based on sequence analysis, protein
homology, and the size of the purified protein. [provided by
RefSeq].1) Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Immunogen
This GTF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-334 amino acids from the C-terminal region of human GTF3A.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.