Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Product Name | GTPBP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTPBP4, CRFG, FLJ10686, FLJ10690, FLJ39774, NGB, NOG1 |
| Product description | GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.1) Lee, H., (2007) Mol. Cell. Biol. 27 (6), 2103-2119. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTPBP4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GTPBP4 antibody can be used for detection of GTPBP4 by ELISA at 1:2500. GTPBP4 antibody can be used for detection of GTPBP4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTPBP4 |
|---|---|
| Alternative Names | GTPBP4 CRFG FLJ10686 FLJ10690 FLJ39774 NGB NOG1 |
| Molecular Weight(MW) | 74 kDa |
Application
Antibody used in WB on Human MCF7 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | GTPBP4 antibody can be used for detection of GTPBP4 by ELISA at 1:2500. GTPBP4 antibody can be used for detection of GTPBP4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTPBP4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
