Application
Western blot analysis in Ramos cell line lysates (35ug/lane).Application
GUCY2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH19| Product Name | GUCY2D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases. [provided by RefSeq].1) Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010) |
| Immunogen | This GUCY2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-570 amino acids from the Central region of human GUCY2D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GUCY2D |
|---|---|
| Alternative Names | Retinal guanylyl cyclase 1 RETGC-1 Guanylate cyclase 2D retinal Rod outer segment membrane guanylate cyclase ROS-GC GUCY2D CORD6 GUC1A4 GUC2D RETGC RETGC1 |
| Molecular Weight(MW) | 120 kDa |
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).
Application
GUCY2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH19| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
