Application
Western blot analysis of GZF1 in human heart tissue lysate with GZF1 antibody at 1 μg/mL.Application
Immunofluorescence of GZF1 in human kidney tissue with GZF1 antibody at 20 μg/mL.Application
Immunohistochemistry of GZF1 in human kidney tissue with GZF1 antibody at 2.5 μg/mL.| Product Name | GZF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GZF1 Antibody: ZBTB23, ZNF336, ZBTB23, GDNF-inducible zinc finger protein 1, Zinc finger and BTB domain-containing protein 23 |
| Product description | GZF1 Antibody: The GDNF-inducible zinc finger protein 1 (GZF1) is a sequence-specific transcriptional repressor with a BTB/POZ domain and ten zinc finger motifs whose expression is required for renal branching morphogenesis during kidney development. GZF1 binds to the 5'regulatory region of the homeodomain protein HOXA10, suggesting that GZF1 may play a role in morphogenesis other than kidney development. Recent experiments have indicated that GZF1 associates with nucleolin and this association is mediated by the first four zinc finger motifs of GZF1. It is thought that Nucleolin modulates the subcellular localization of GZF1 as well as its transcriptional repressor activity.1) Fukuda N, Ichihara M, Morinaga T, et al. Identification of a novel glial cell line-derived neurotrophic factor-inducible gene required for renal branching morphogenesis. J. Biol. Chem. 2003; 278:50386-92 |
| Immunogen | GZF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GZF1. The immunogen is located within amino acids 600 - 650 of GZF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GZF1 |
|---|---|
| Alternative Names | GZF1 Antibody: ZBTB23 ZNF336 ZBTB23 GDNF-inducible zinc finger protein 1 Zinc finger and BTB domain-containing protein 23 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | GZF1 antibody is human specific. At least four isoforms of GZF1 are known to exist. |
Application
Western blot analysis of GZF1 in human heart tissue lysate with GZF1 antibody at 1 μg/mL.
Application
Immunofluorescence of GZF1 in human kidney tissue with GZF1 antibody at 20 μg/mL.
Application
Immunohistochemistry of GZF1 in human kidney tissue with GZF1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | GZF1 antibody can be used for detection of GZF1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GZF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GZF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
