Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | H1FX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone H1x, H1FX |
| Product description | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family.1) Warneboldt, J., et al. BMC Cancer 8, 388 (2008) : |
| Immunogen | This H1FX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the C-terminal region of human H1FX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1FX |
|---|---|
| Alternative Names | Histone H1x H1FX |
| Molecular Weight(MW) | 22 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
