Application
Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.| Product Name | H2BFWT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the H2B histone family that is specifically expressed in sperm nuclei. A polymorphism in the 5' UTR of this gene is associated with male infertility.1) Lee, J., et al. J. Cell. Mol. Med. 13 (8B), 1942-1951 (2009) : |
| Immunogen | This H2BFWT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-88 amino acids from the Central region of human H2BFWT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2BFWT |
|---|---|
| Alternative Names | Histone H2B type W-T H2B histone family member W testis-specific H2BFWT |
| Molecular Weight(MW) | 20 kDa |
Application
Western Blot at 1:1000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
