Application
Antibody used in WB on Human Brain at 1 ug/ml.| Product Name | H3F3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histone H3, along with histone H4, plays a central role in nucleosome formation.1) Wells, D. and Kedes, L. (1985) Proc. Natl. Acad. Sci. U.S.A. 82: 2834-2838.. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide directed towards the N terminal of human HIST3H3 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| H3F3A antibody can be used for detection of H3F3A by ELISA at >1:3000. H3F3A antibody can be used for detection of H3F3A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIST3H3 |
|---|---|
| Alternative Names | H3F3A H3t H3.4 H3/g H3FT |
| Molecular Weight(MW) | 15 kDa, 15 kDa, 16 kDa, 15 kDa, 23 kDa |
Application
Antibody used in WB on Human Brain at 1 ug/ml.| Application Notes | H3F3A antibody can be used for detection of H3F3A by ELISA at >1:3000. H3F3A antibody can be used for detection of H3F3A by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store H3F3A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
