Application
Western blot analysis of HIST3H3 Antibody (N-term) in CEM, K562, HL-60 cell line lysates (35ug/lane). HIST3H3 (arrow) was detected using the purified Pab.| Product Name | Histone H3.1t antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Histone H3.1tHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human HIST3H3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIST3H3 H3FT H3/t H3/g HIST3H3 Histone H3.1t H3t |
|---|---|
| Target | HIST3H3 |
| Molecular Weight(MW) | 15.508 kDa (note) |
Application
Western blot analysis of HIST3H3 Antibody (N-term) in CEM, K562, HL-60 cell line lysates (35ug/lane). HIST3H3 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
