Application
HIST3H3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST3H3 antibody detected the HIST3H3 protein (arrow).Application
HIST3H3 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | HIST3H3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FT |
|---|---|
| Alternative Names | HIST3H3 H3FT Histone H3.1t H3/g |
| Molecular Weight(MW) | 15508 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | Expressed in testicular cells. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8290 |
|---|---|
| Nucleotide Accession | NP_003484.1 |
Application
HIST3H3 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST3H3 antibody detected the HIST3H3 protein (arrow).
Application
HIST3H3 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM235065