Application
A. Western blot analysis on NIH3T3 cell lysate using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d); dilution 1:2,000. Cell lysates were (1) untreated, or (2) treated with FBS and CalA.Application
B. Immunohistochemical staining of paraffin-embedded lymphoma using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d).Application
C. Immunohistochemical staining of Phosphatase-treated paraffin-embedded lymphoma using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d).| Product Name | Histone H3 Antibody Phospho (pS28) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S28 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E191 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FT |
|---|---|
| Alternative Names | HIST3H3 H3FT Histone H3.1t H3/g |
| Molecular Weight(MW) | 15508 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | Expressed in testicular cells. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8290 |
|---|
Application
A. Western blot analysis on NIH3T3 cell lysate using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d); dilution 1:2,000. Cell lysates were (1) untreated, or (2) treated with FBS and CalA.
Application
B. Immunohistochemical staining of paraffin-embedded lymphoma using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d).
Application
C. Immunohistochemical staining of Phosphatase-treated paraffin-embedded lymphoma using anti- Phospho-Histone H3 (pS28) RabMAb (Cat. #AJ1353d).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM235084