Application
A. Western blot analysis using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d); dilution 1:20,000. Lane (1) Hela cell lysate; lane (2) recombinant Histone H3 (non-methylated).Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d).Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d).| Product Name | Histone-H3 Antibody Dimethyl (K4) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | Dimethyl K4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y47 |
| Host Species | Rabbit |
| Tested Applications | WBIHCIF |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3FT |
|---|---|
| Alternative Names | HIST3H3 H3FT Histone H3.1t H3/g |
| Molecular Weight(MW) | 15508 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | Expressed in testicular cells. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8290 |
|---|
Application
A. Western blot analysis using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d); dilution 1:20,000. Lane (1) Hela cell lysate; lane (2) recombinant Histone H3 (non-methylated).
Application
B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d).
Application
C. Immunofluorescent staining of HeLa cells using anti-Histone H3 Dimethyl (K4) RabMAb (Cat. #AJ1357d).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM235091