Application
Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 μg/mL.Application
Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 μg/mL.Application
Immunofluorescence of HAAO in Human Liver cells with HAAO antibody at 20 μg/mL.| Product Name | HAAO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HAAO Antibody: HAO, 3-HAO, 3-hydroxyanthranilate 3, 4-dioxygenase, 3-hydroxyanthranilate oxygenase |
| Product description | HAAO Antibody: HAAO (3-Hydroxyanthranilate 3, 4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system. This enzyme participates in tryptophan metabolism. It employs one cofactor, iron. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.1) Decker RH, Kang HH, Leach FR, et al. Purification and properties of 3-hydroxyanthranilic acid oxidase. J. Biol. Chem.1961; 236:3076-82. |
| Immunogen | HAAO antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human HAAO. The immunogen is located within amino acids 30 - 80 of HAAO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HAAO antibody can be used for detection of HAAO by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HAAO |
|---|---|
| Alternative Names | HAAO Antibody: HAO 3-HAO 3-hydroxyanthranilate 3 4-dioxygenase 3-hydroxyanthranilate oxygenase |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of HAAO in human liver tissue with HAAO antibody at 2.5 μg/mL.
Application
Western blot analysis of HAAO in Mouse liver tissue lysate with HAAO antibody at 1 μg/mL.
Application
Immunofluorescence of HAAO in Human Liver cells with HAAO antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | HAAO antibody can be used for detection of HAAO by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | HAAO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HAAO Antibody is supplied in PBS containing 0.02% sodium azide. |
