HAMP is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity.1) Matsumoto, M., et al. Circ. J. 74(2):301-306(2010)
Immunogen
This HAMP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the Central region of human HAMP.
Western blot analysis of HAMP Antibody in MDA-MB435 cell line lysates (35ug/lane)
Application
Western Blot at 1:500 dilution + human liver lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:500
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.