Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-HAND2 Antibody in NCI-H460 cell line lysates (35ug/lane).| Product Name | HAND2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HAND2 belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.1) McFadden, D.G., et al., Development 127(24):5331-5341 (2000). Firulli, A.B., et al., Nat. Genet. 18(3):266-270 (1998). Russell, M.W., et al., Biochim. Biophys. Acta 1443(3):393-399 (1998). Srivastava, D., Trends Cardiovasc Med(1-2), 11-18 (1999). Srivastava, D., et al., Cold Spring Harb. Symp. Quant. Biol. 67, 121-125 (2002). |
| Immunogen | This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-207 amino acids from the C-terminal region of human HAND2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | HAND2 |
|---|---|
| Alternative Names | Heart- and neural crest derivatives-expressed protein 2 Class A basic helix-loop-helix protein 26 bHLHa26 Deciduum heart autonomic nervous system and neural crest derivatives-expressed protein 2 dHAND HAND2 BHLHA26 DHAND |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-HAND2 Antibody in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
