Application
Western blot analysis of HAP1 in mouse brain tissue lysate with HAP1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of HAP1 in Human Brain cells with HAP1 antibody at 20 μg/mL.Application
Immunohistochemistry of HAP1 in human brain tissue with HAP1 antibody at 2.5 μg/mL.| Product Name | HAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HAP1 Antibody: HLP, HAP2, HIP5, hHLP1, HLP1, Huntingtin-associated protein 1, Neuroan 1, HAP-1 |
| Product description | HAP1 Antibody: Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. HAP1 was initially identified through a two-hybrid library screening; the binding of HAP1 to huntingtin correlated with the expansion of the polyglutamine tract. HAP1 also interacts with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), suggesting that HAP1 may play a role in vesicular trafficking or organelle transport. HAP1 is also involved with the huntingtin-enhanced BDNF transport along the cellular microtubles. Attenuation of this process led to the loss of neurotrophic support and neuronal toxicity, which suggests that loss of this function might contribute to pathogenesis. Several alternatively spliced isoforms have been described for HAP1.1) Borrell-Pagès M, Zala D, Humbert S, et al. Huntington's disease: from huntingtin function and dysfunction to therapeutic strategies. Cell Mol. Life Sci.2006; 63:2642-60. |
| Immunogen | HAP1 antibody was raised against a 19 amino acid synthetic peptide from near the center of human HAP1. The immunogen is located within amino acids 310 - 360 of HAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HAP1 antibody can be used for detection of HAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HAP1 |
|---|---|
| Alternative Names | HAP1 Antibody: HLP HAP2 HIP5 hHLP1 HLP1 Huntingtin-associated protein 1 Neuroan 1 HAP-1 |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of HAP1 in mouse brain tissue lysate with HAP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of HAP1 in Human Brain cells with HAP1 antibody at 20 μg/mL.
Application
Immunohistochemistry of HAP1 in human brain tissue with HAP1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | HAP1 antibody can be used for detection of HAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | HAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
