Application
Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | HARS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS |
| Product description | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.1) Levine,S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007) |
| Immunogen | This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HARS |
|---|---|
| Alternative Names | Histidine--tRNA ligase cytoplasmic Histidyl-tRNA synthetase HisRS HARS HRS |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
