Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
Western blot analysis of HBG1 using rabbit polyclonal HBG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HBG1 gene.| Product Name | HBG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.1) Zhou, D., et al. Nat. Genet. 42(9):742-744(2010) |
| Immunogen | This HBG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the Central region of human HBG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HBG1 |
|---|---|
| Alternative Names | Hemoglobin subunit gamma-1 Gamma-1-globin Hb F Agamma Hemoglobin gamma-1 chain Hemoglobin gamma-A chain HBG1 |
| Molecular Weight(MW) | 16 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
Western blot analysis of HBG1 using rabbit polyclonal HBG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HBG1 gene.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
