Application
Western blot analysis of lysates from K562 cell line and human placenta tissue lysate (from left to right), using HBG2 Antibody . was diluted at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Product Name | HBG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.1) Slightom J.L.,et al.Cell 21:627-638(1980). |
| Immunogen | This HBG2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-133 amino acids from the C-terminal region of human HBG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HBG2 |
|---|---|
| Alternative Names | Hemoglobin subunit gamma-2 Gamma-2-globin Hb F Ggamma Hemoglobin gamma-2 chain Hemoglobin gamma-G chain HBG2 |
| Molecular Weight(MW) | 16 kDa |
Application
Western blot analysis of lysates from K562 cell line and human placenta tissue lysate (from left to right), using HBG2 Antibody . was diluted at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded M. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of U-87 MG cells using HBG2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H. liver section using HBG2 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
