Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.| Product Name | HCC-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hHCC-1 human Macrophage-Derived Chemokine). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: To detect hHCC-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hHCC-1. Sandwich: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human HCC-1 (XP-5151Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL14 |
|---|---|
| Alternative Names | CC-1 CC-3 CKB1 MCIF NCC2 SY14 HCC-1 HCC-3 NCC-2 SCYL2 SCYA14 HCC-1(1-74) HCC-1/HCC-3 C-C motif chemokine 14 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary.| Application Notes | ELISA: To detect hHCC-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hHCC-1. Sandwich: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human HCC-1 (XP-5151Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | HCC-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
