Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | HCLS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.1) Ruzzene, M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCLS1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:2500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HCLS1 |
|---|---|
| Alternative Names | HCLS1 HS1 CTTNL |
| Molecular Weight(MW) | 54 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:2500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCLS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
