WB
Western blot with Anti-Phospho-HCLS1(Tyr378) antibody on lysates of HepG2 cell (1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 3% BSA for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Product Name | Anti-Phospho-HCLS1 (Tyr378) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH |
| Modification | p-Tyr378 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | HCLS1 |
|---|---|
| Gene Synonyms | HS1 p75 CTTNL lckBP1 |
| Gene Full Name | hematopoietic cell-specific Lyn substrate 1 |
| Gene Summary | Enables RNA polymerase II-specific DNA-binding transcription factor binding activity; protein kinase binding activity; and signaling adaptor activity. Involved in several processes, including granulocyte colony-stimulating factor signaling pathway; positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and positive regulation of protein import into nucleus. Located in cytosol; nucleus; and plasma membrane. Part of transcription regulator complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Cortactin like CTTNL HCLS 1 Hcls1 HCLS1_HUMAN Hematopoietic cell specific Lyn substrate 1 Hematopoietic cell-specific LYN substrate 1 Hematopoietic lineage cell-specific protein HS 1 HS1 LckBP1 OTTHUMP00000215180 OTTHUMP00000215182 p75 |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Nucleus. Nucleus>Nucleoplasm. Nucleus matrix. Chromosome. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cell cortex. Cell membrane>Lipid-anchor>Cytoplasmic side. Lateral cell membrane. |
| SwissProt ID | P14317 |
|---|
WB
Western blot with Anti-Phospho-HCLS1(Tyr378) antibody on lysates of HepG2 cell (1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) containing 3% BSA for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 120S.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
