Application
Western blot analysis of lysed extracts from HeLa, K562 and 3T3 cells using HDAC3 Antibody.| Product Name | HDAC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation |
| Immunogen | HDAC3 antibody was raised against a peptide sequence around aa.420~424 (N-D-K-E-S) derived from Human HDAC3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC3 |
|---|---|
| Alternative Names | HD3 RPD3 RPD3-2 HD3 |
| Molecular Weight(MW) | 49 kDa |
| Tissue Specificity | This antibody detects endogenous level of total HDAC3 protein. |
| Entrez Gene | 8841 |
|---|---|
| Protein Accession | NP_003874.2 |
Application
Western blot analysis of lysed extracts from HeLa, K562 and 3T3 cells using HDAC3 Antibody.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
