WB
Western blot analysis of various lysates using HDAC3 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat thymus tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of HeLa cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.ICC/IF
Confocal imaging of C6 cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | HDAC3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:3000 - 1:10000 IHC:1:500 - 1:2000 ICC/IF:1:100 - 1:200 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC3 |
|---|---|
| Gene Synonyms | HD3 RPD3 KDAC3 RPD3-2 |
| Gene Full Name | histone deacetylase 3 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | HD3 RPD3 KDAC3 RPD3-2 |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Cytoplasm, Nucleus, cytosol. |
WB
Western blot analysis of various lysates using HDAC3 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat thymus tissue using HDAC3 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of HeLa cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of C6 cells using HDAC3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:3000 - 1:10000 IHC:1:500 - 1:2000 ICC/IF:1:100 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
