WB
Western blot analysis of lysates from NIH/3T3 cells, using HDAC3 (Phospho-Ser424) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human skin, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.ICC/IF
Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | HDAC3 (Phospho Ser424) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HDAC3 around the phosphorylation site of Ser424. AA range:379-428 |
| Modification | p-Ser424 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC3 |
|---|---|
| Gene Synonyms | HD3 RPD3 KDAC3 RPD3-2 |
| Gene Full Name | histone deacetylase 3 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | HDAC3 Histone deacetylase 3 HD3 RPD3-2 SMAP45 |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus . Cytoplasm . Cytoplasm , cytosol . Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803) . Predominantly expressed in the nucleus in the presence of CCAR2 (PubMed:21030595) . . |
WB
Western blot analysis of lysates from NIH/3T3 cells, using HDAC3 (Phospho-Ser424) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human skin, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.
ICC/IF
Immunofluorescence analysis of A549 cells, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
