Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (Phospho-Ser246/259/220).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220).| Product Name | HDAC4 / HDAC5 / HDAC9 (phospho Ser246 / Ser259 / Ser220) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.1) Cress, W. D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Vigushin, D. M. and Coombes, R. C. (2004) Curr. Cancer Drug Targets 4, 205-218. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100. |
| Immunogen | HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody was raised against a peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S (p) -EP) derived from Human HDAC4/HDAC5/HDAC9. |
| Modification | p-Ser246 / Ser259 / Ser220 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC4 HDAC5 HDAC9 |
|---|---|
| Alternative Names | HD4 AHO3 BDMR HDACA HA6116 HDAC-4 HDAC-A CYK18 PIG46 HD4/HD5/HD9 |
| Molecular Weight(MW) | 140, 124 kDa |
| Tissue Specificity | This antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220. |
| Entrez Gene | 9759 / 10014 / 9734 |
|---|---|
| Protein Accession | NP_006028.2 / NP_001015053.1 / NP_055522.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (Phospho-Ser246/259/220).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
