Application
Antibody used in CHIP on HCT116 Cells.Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | HDAC6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.1) Int J Mol Med. 2003 Jul;12(1):87-93.. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HDAC6 antibody can be used for detection of HDAC6 by ELISA at 1:62500. HDAC6 antibody can be used for detection of HDAC6 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC6 |
|---|---|
| Alternative Names | HDAC6 HD6 JM21 CPBHM |
| Molecular Weight(MW) | 16 kDa, 131 kDa |
Application
Antibody used in CHIP on HCT116 Cells.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | HDAC6 antibody can be used for detection of HDAC6 by ELISA at 1:62500. HDAC6 antibody can be used for detection of HDAC6 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HDAC6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
