Application
Immunofluorescence staining of methanol-fixed HeLa cells using HDAC8 (Phospho-Ser39).Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8 (Phospho-Ser39).| Product Name | HDAC8 (phospho Ser39) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC8 gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.1) Lee H, et al. (2004) Mol Cell Biol. 24 (2): 765-773. |
| Immunogen | HDAC8 (Phospho-Ser39) antibody was raised against a peptide sequence around phosphorylation site of serine 39 (R-A-S (p) -M-V) derived from Human HDAC8. |
| Modification | p-Ser39 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC8 |
|---|---|
| Alternative Names | HD8 WTS RPD3 CDA07 CDLS5 MRXS6 HDACL1 MAPKAPK1A RSK1 HD8 HDA8 |
| Molecular Weight(MW) | 42 kDa |
| Tissue Specificity | This antibody detects endogenous level of HDAC8 only when phosphorylated at serine 39. |
| Entrez Gene | 55869 |
|---|---|
| Protein Accession | NP_001159890.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using HDAC8 (Phospho-Ser39).
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HDAC8 (Phospho-Ser39).| Application Notes | Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
