Application
Western blot analysis of HDGFRP2 in rat lung tissue lysate with HDGFRP2 antibody at 2 μg/mL .Application
Immunofluorescence of HDGFRP2 in rat liver tissue with HDGFRP2 antibody at 20 μg/mL.Application
Immunohistochemistry of HDGFRP2 in rat liver tissue with HDGFRP2 antibody at 5 μg/mL.| Product Name | HDGFRP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HDGFRP2 Antibody: Hepatoma-derived growth factor-related protein 2 (HDGFRP2) is a widely expressed member of protein family that contains the HDGF related proteins 1-4 and lens epithelial derived growth factor (LEDGF). These family members show extensive homology at the amino terminus (termed the hath region). Little is known of the role of HDGFRP2, but like the related LEDGF, HDGFRP2 can bind HIV-1 integrase and stimulate HIV-1 integrase activity in vitro.1) El-Tahir HM, Dietz F, Dringen R, et al. Expression of Hepatoma-derived growth factor family members in the adult central nervous system. BMC Neurosci. 2006; 7:6. |
| Immunogen | HDGFRP2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human HDGFRP2. The immunogen is located within amino acids 50 - 100 of HDGFRP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HDGFRP2 antibody can be used for detection of HDGFRP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDGFRP2 |
|---|---|
| Alternative Names | HDGFRP2 Antibody: HDGF2 HDGF2 UNQ785/PRO1604 Hepatoma-derived growth factor-related protein 2 Hepatoma-derived growth factor 2 HRP-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (94%) |
| Tissue Specificity | At least two isoforms of HDGFRP2 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other HDGFRP family members |
Application
Western blot analysis of HDGFRP2 in rat lung tissue lysate with HDGFRP2 antibody at 2 μg/mL .
Application
Immunofluorescence of HDGFRP2 in rat liver tissue with HDGFRP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of HDGFRP2 in rat liver tissue with HDGFRP2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | HDGFRP2 antibody can be used for detection of HDGFRP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HDGFRP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HDGFRP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
