Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | HELT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HELT belongs to the HEY family. It contains 1 basic helix-loop-helix (bHLH) domain and 1orange domain. HELT is a transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGCG-3'.1) Guimera, J., Gene 377, 65-76 (2006). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HELT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HELT antibody can be used for detection of HELT by ELISA at 1:312500. HELT antibody can be used for detection of HELT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HELT |
|---|---|
| Alternative Names | HELT HCM1228 HESL Mgn bHLHb44 |
| Molecular Weight(MW) | 36 kDa |
| Entrez Gene | 391723 |
|---|---|
| Protein Accession | NP_001025058 |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | HELT antibody can be used for detection of HELT by ELISA at 1:312500. HELT antibody can be used for detection of HELT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HELT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
