Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | HERC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This HERC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-77 amino acids from the N-terminal region of human HERC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HERC3 |
|---|---|
| Alternative Names | Probable E3 ubiquitin-protein ligase HERC3 632- HECT domain and RCC1-like domain-containing protein 3 HERC3 KIAA0032 |
| Molecular Weight(MW) | 117 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
