Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HFM1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Product Name | HFM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This HFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1293-1321 amino acids from the C-terminal region of human HFM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HFM1 |
|---|---|
| Alternative Names | Probable ATP-dependent DNA helicase HFM1 SEC63 domain-containing protein 1 HFM1 SEC3D1 |
| Molecular Weight(MW) | 163 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HFM1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
