Application
Immunohistochemistry of HHATL in human skin tissue with HHATL antibody at 2.5 μg/mL.Application
Immunofluorescence of HHATL in human skin tissue with HHATL antibody at 20 μg/mL.Application
Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 μg/mL.| Product Name | HHATL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.1) Abe Y, Kita Y, and Niikura T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-palmitoylation of Sonic hedgehog. FEBS J. 2008; 275:318-31. |
| Immunogen | HHATL antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HHATL. The immunogen is located within the first 50 amino acids of HHATL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| HHATL antibody can be used for detection of HHATL by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HHATL |
|---|---|
| Alternative Names | HHATL Antibody: GUP1 OACT3 C3orf3 MBOAT3 MSTP002 GUP1 KIAA1173 Protein-cysteine N-palmitoyltransferase HHAT-like protein Hedgehog acyltransferase-like protein |
| Sequence Similarities | 2) Cat. No. 12-701 - Human Skin Tissue Slide |
Application
Immunohistochemistry of HHATL in human skin tissue with HHATL antibody at 2.5 μg/mL.
Application
Immunofluorescence of HHATL in human skin tissue with HHATL antibody at 20 μg/mL.
Application
Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | HHATL antibody can be used for detection of HHATL by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HHATL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HHATL Antibody is supplied in PBS containing 0.02% sodium azide. |
