Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of HIBCH Antibody in K562 cell line lysates (35ug/lane)(2ug/ml)| Product Name | HIBCH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, 3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH |
| Product description | HIBCH is responsible for the specific hydrolysis of HIBYL-CoA, a valine catabolite, as well as the hydrolysis of beta-hydroxypropionyl-CoA, an intermediate in a minor pathway of propionate metabolism.1) Hawes J.W., Jaskiewicz J., Shimomura Y.J. Biol. Chem. 271:26430-26434(1996) |
| Immunogen | This HIBCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-209 amino acids from the Central region of human HIBCH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | HIBCH |
|---|---|
| Alternative Names | 3-hydroxyisobutyryl-CoA hydrolase mitochondrial 3-hydroxyisobutyryl-coenzyme A hydrolase HIB-CoA hydrolase HIBYL-CoA-H HIBCH |
| Molecular Weight(MW) | 43 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIBCH antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of HIBCH Antibody in K562 cell line lysates (35ug/lane)(2ug/ml)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
