Application
Immunocytochemistry of HIG1 in 293 cells with HIG1 antibody at 2.5 μg/mL.Application
Western blot analysis of HIG1 in 293 cell lysate with HIG1 antibody at at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of HIG1 in 293 cells tissue with HIG1 antibody at 10 μg/mL.| Product Name | HIG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIG1 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.1) Bedo G, Vargas M, Ferreiro MJ, et al. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int. J. Dev. Biol. 2005; 49:431-6. |
| Immunogen | HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1. The immunogen is located within the first 50 amino acids of HIG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIGD1A |
|---|---|
| Alternative Names | HIG1 Antibody: HIG1 RCF1a HIG1 HSPC010 HIG1 domain family member 1A mitochondrial Hypoxia-inducible gene 1 protein |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | HIG1 antibody is predicted to not cross-react with HIG2 |
| Entrez Gene | 25994 |
|---|---|
| Protein Accession | NP_001093138 |
Application
Immunocytochemistry of HIG1 in 293 cells with HIG1 antibody at 2.5 μg/mL.
Application
Western blot analysis of HIG1 in 293 cell lysate with HIG1 antibody at at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of HIG1 in 293 cells tissue with HIG1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HIG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HIG1 Antibody is supplied in PBS containing 0.02% sodium azide. |
