Application
Western blot analysis of HIG2 in A549 cell lysate with HIG2 antibody at 1 μg/mL.| Product Name | HIG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIG2 Antibody: HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG2 is induced by hypoxia and by glucose deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cancer cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis.1) Bedo G, Vargas M, Ferreiro MJ, et al. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int. J. Dev. Biol. 2005; 49:431-6 |
| Immunogen | HIG2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human HIG2. The immunogen is located within the last 50 amino acids of HIG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HIG2 antibody can be used for detection of HIG2 by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIGD2A |
|---|---|
| Alternative Names | HIG2 Antibody: RCF1b HIG1 domain family member 2A mitochondrial RCF1 homolog B RCF1b |
| Tissue Specificity | At least two isoforms of HIG2 are known to exist; this antibody will detect both isoforms. HIG2 antibody is predicted to not cross-react with HIG1 |
| Entrez Gene | 192286 |
|---|---|
| Protein Accession | NP_001092256 |
Application
Western blot analysis of HIG2 in A549 cell lysate with HIG2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | HIG2 antibody can be used for detection of HIG2 by Western blot at 1 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | HIG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | HIG2 Antibody is supplied in PBS containing 0.02% sodium azide. |
