Application
Antibody used in WB on HepG2 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 0.1 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.06 ug/ml.Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Product Name | HIPK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.1) Kim, E.A., (2006) J. Biol. Chem. 281 (11), 7489-7497. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIPK2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HIPK2 antibody can be used for detection of HIPK2 by ELISA at 1:62500. HIPK2 antibody can be used for detection of HIPK2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIPK2 |
|---|---|
| Alternative Names | HIPK2 PRO0593 |
| Molecular Weight(MW) | 89 kDa, 128 kDa, 28 kDa, 51 kDa |
Application
Antibody used in WB on HepG2 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.06 ug/ml.
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | HIPK2 antibody can be used for detection of HIPK2 by ELISA at 1:62500. HIPK2 antibody can be used for detection of HIPK2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HIPK2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
