Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-HIPK2 Antibody in Ramos cell line lysates (35ug/lane).| Product Name | HIPK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | HIPK2, a member of the KIPK subfamily of Ser/Thr protein kinases, phosphorylates homeodomain transcription factors and may play a role as a corepressor for homeodomain transcription factors. This nuclear protein has been shown to interact with TRADD. It is highly expressed in neuronal tissues, heart and kidney, and weakly expressed in a ubiquitous way. HIPK2 is a target for sumoylation, and when conjugated it is directed to nuclear speckles.1) Li, X., et al., Biochem. Biophys. Res. Commun. 277(2):513-517 (2000). |
| Immunogen | This HIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-468 amino acids from the center region of human HIPK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIPK2 |
|---|---|
| Alternative Names | Homeodomain-interacting protein kinase 2 hHIPk2 HIPK2 |
| Molecular Weight(MW) | 131 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-HIPK2 Antibody in Ramos cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
