Application
Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody . was diluted at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HIST1H2AG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP |
| Product description | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.1) Albig W.,et al.Hum. Genet. 101:284-294(1997). |
| Immunogen | This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIST1H2AG |
|---|---|
| Alternative Names | Histone H2A type 1 H2A1 Histone H2A/p HIST1H2AG H2AFP |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of lysates from Hela, HepG2, HL-60 cell line (from left to right), using HIST1H2AG Antibody . was diluted at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: L929 whole cell lysate Lane 6: MCF-7 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
