Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2BH/HIST1H2BK/HIST3H2BB antibody detected the HIST1H2BH/HIST1H2BK/HIST3H2BB protein (arrow).| Product Name | HIST1H2BH/HIST1H2BK/HIST3H2BB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone H2B type 1-H, Histone H2Bj, H2B/j, HIST1H2BH, H2BFJ |
| Product description | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].1) Kim, S.C., et al. Mol. Cell 23(4):607-618(2006) |
| Immunogen | This HIST1H2BH/HIST1H2BK/HIST3H2BB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-52 amino acids from the Central region of human HIST1H2BH/HIST1H2BK/HIST3H2BB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HIST1H2BH |
|---|---|
| Alternative Names | Histone H2B type 1-H Histone H2Bj H2B/j HIST1H2BH H2BFJ |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Xenopus |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2BH/HIST1H2BK/HIST3H2BB antibody detected the HIST1H2BH/HIST1H2BK/HIST3H2BB protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
