Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | HIST2H2AA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIST2H2AA3, H2A, H2A.2, H2A/O, H2A/q, H2AFO, H2a-615, HIST2H2AA |
| Product description | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. HIST2H2AA3 is a member of the histone H2A family.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Gregory, S.G., (2006) Nature 441 (7091), 315-321. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HIST2H2AA3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HIST2H2AA3 antibody can be used for detection of HIST2H2AA3 by ELISA at 1:12500. HIST2H2AA3 antibody can be used for detection of HIST2H2AA3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HIST2H2AA3 |
|---|---|
| Alternative Names | HIST2H2AA3 H2A H2A.2 H2A/O H2A/q H2AFO H2a-615 HIST2H2AA |
| Molecular Weight(MW) | 14 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HIST2H2AA3 antibody can be used for detection of HIST2H2AA3 by ELISA at 1:12500. HIST2H2AA3 antibody can be used for detection of HIST2H2AA3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HIST2H2AA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
