Application
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X (Phospho-Ser139).Application
Western blot analysis of lysed extracts from HT29 cells untreated (Lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139).| Product Name | Histone H2A.X (phospho Ser139) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.1) Yaneva M, et al. (2005) Nucleic Acids Res. 33 (16): 5320-5330. Tsukuda T, et al. (2006) Nature. Author manuscript; available in PMC 2006 March 6. |
| Immunogen | Histone H2A.X (Phospho-Ser139) antibody was raised against a peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X. |
| Modification | p-Ser139 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AFX |
|---|---|
| Alternative Names | H2AX H2A.X H2A/X HNF3B TCF3B H2AFX H2a/x HIST5-2AX |
| Molecular Weight(MW) | 15 kDa |
| Tissue Specificity | This antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139. |
| Entrez Gene | 3014 |
|---|---|
| Protein Accession | NP_002096.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Histone H2A.X (Phospho-Ser139).
Application
Western blot analysis of lysed extracts from HT29 cells untreated (Lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM280979