HKR1 is one of the GLI-Kruppel family of human genes. Members of this family have similar H-C links, i.e., a conserved stretch of 9 amino acids connecting the C-terminal histidine of one finger to the N-terminal cysteine of the next. On the basis of amino acid sequence and intron-exon organization, the genes could be placed into one of two subgroups: the GLI subgroup (with the consensus finger amino acid sequence [Y/F]XCX3GCX3[F/Y]X5LX2HX3-4H[T/S]GEKP) or the Kr subgroup (with the consensus finger amino acid sequence [Y/F]XCX2CX3FX5LX2HXRXHTGEKP).1) Suzuki, Y., Gene 200 (1-2), 149-156 (1997).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HKR1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
HKR1 antibody can be used for detection of HKR1 by ELISA at 1:12500. HKR1 antibody can be used for detection of HKR1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.125 ug/ml.
Application
Antibody used in IHC on Human Heart.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
HKR1 antibody can be used for detection of HKR1 by ELISA at 1:12500. HKR1 antibody can be used for detection of HKR1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HKR1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.