Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Product Name | HLA-DMB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein, HLA-DMB, DMB, RING7 |
| Product description | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.1) Rinderknecht, C.H., et al. Immunology 131(1):18-32(2010) |
| Immunogen | This HLA-DMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human HLA-DMB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-DMB |
|---|---|
| Alternative Names | HLA class II histocompatibility antigen DM beta chain MHC class II antigen DMB Really interesting new gene 7 protein HLA-DMB DMB RING7 |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
