Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of HLA-DQA1 using rabbit polyclonal HLA-DQA1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DQA1 gene.Application
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | HLA-DQA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1 |
| Product description | HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.1) Donaldson, P.T., et al. J. Hepatol. 53(6):1049-1053(2010) |
| Immunogen | This HLA-DQA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the N-terminal region of human HLA-DQA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-DQA1 |
|---|---|
| Alternative Names | HLA class II histocompatibility antigen DQ alpha 1 chain DC-1 alpha chain DC-alpha HLA-DCA MHC class II DQA1 HLA-DQA1 |
| Molecular Weight(MW) | 28 kDa |
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of HLA-DQA1 using rabbit polyclonal HLA-DQA1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DQA1 gene.
Application
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
