Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.| Product Name | HLA-F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA-F, CDA12, HLA-5.4, HLA-CDA12, HLAF |
| Product description | HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.1) Burfoot, R.K., (2008) Tissue Antigens 71 (1), 42-50. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HLA-F. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HLA-F antibody can be used for detection of HLA-F by ELISA at 1:12500. HLA-F antibody can be used for detection of HLA-F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-F |
|---|---|
| Alternative Names | HLA-F CDA12 HLA-5.4 HLA-CDA12 HLAF |
| Molecular Weight(MW) | 48 kDa |
| Entrez Gene | 3134 |
|---|---|
| Protein Accession | NP_001091949 |
Application
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | HLA-F antibody can be used for detection of HLA-F by ELISA at 1:12500. HLA-F antibody can be used for detection of HLA-F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HLA-F antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
