Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | HLA-F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HLA class I histocompatibility antigen, alpha chain F, CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F, HLA-F, HLA-54, HLAF |
| Immunogen | This HLA-F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-198 amino acids from the Central region of human HLA-F. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-F |
|---|---|
| Alternative Names | HLA class I histocompatibility antigen alpha chain F CDA12 HLA F antigen Leukocyte antigen F MHC class I antigen F HLA-F HLA-54 HLAF |
| Molecular Weight(MW) | 39 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
