Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | HMBOX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMBOX1, HOT1, PBHNF, HNF1LA |
| Product description | HMBOX1 is located at the boundary of 8p12.3 and 8p21.1. HMBOX1 proteins are highly conserved in human, mouse, rat, chicken and Xenopus laevis. Functional HMBOX1::EGFP (enhanced green fluorescent protein) fusion protein revealed that HMBOX1 accumulated more in cytoplasm than in nucleus. HMBOX1 is a transcription repressor. Human HMBOX1 is widely expressed in 18 tissues, and it is highly expressed in pancreas. Hmbox1 is widely expressed in mouse pancreas and the expression of this gene can also be detected in pallium, hippocampus and hypothalamus.1) Strausberg, R.L., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMBOX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HMBOX1 antibody can be used for detection of HMBOX1 by ELISA at 1:312500. HMBOX1 antibody can be used for detection of HMBOX1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HMBOX1 |
|---|---|
| Alternative Names | HMBOX1 HOT1 PBHNF HNF1LA |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HMBOX1 antibody can be used for detection of HMBOX1 by ELISA at 1:312500. HMBOX1 antibody can be used for detection of HMBOX1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMBOX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
