The human HMG2a gene is transcribed mainly in the placenta. HMG2a encodes a protein which is part of the high mobility group (HMG) family. Members of this family are ubiquitously expressed and facilitate the formation of nucleoprotein complexes where the DNA is sharply bent.1) Vaccari, T., et al., (1998) Genomics 49 (2), 247-252.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
HMGB3 antibody can be used for detection of HMGB3 by ELISA at 1:312500. HMGB3 antibody can be used for detection of HMGB3 by western blot at 0.3 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Placenta at 0.3 ug/ml.
Application
Antibody used in IHC on Human Intestine.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
HMGB3 antibody can be used for detection of HMGB3 by ELISA at 1:312500. HMGB3 antibody can be used for detection of HMGB3 by western blot at 0.3 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HMGB3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt