Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).| Product Name | HMGB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | High mobility group protein B3, High mobility group protein 2a, HMG-2a, High mobility group protein 4, HMG-4, HMGB3, HMG2A, HMG4 |
| Product description | HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).1) Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006) |
| Immunogen | This HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-79 amino acids from the Central region of human HMGB3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HMGB3 |
|---|---|
| Alternative Names | High mobility group protein B3 High mobility group protein 2a HMG-2a High mobility group protein 4 HMG-4 HMGB3 HMG2A HMG4 |
| Molecular Weight(MW) | 23 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
